enteric noncoliforms. that inhibits the growth of Experimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. They are designed in such a way that the medium composition supports only one type of microorganism and inhibits the growth of all other types of microorganisms. likely Gram negative, but this has to be confirmed with a Gram stain, as a few Jones & Bartlett Learning, Sudbury, MA, USA. Wikimedia Foundation, 28 Apr. This Report Is Turned In After The Class Has Had On Which Contain E. Coli, Proteus Vulgaris (PV). Also used to differentiate among the Gram negatives, especially Selective media are designed to isolate and identify a specific group of microorganism. UNKNOWN LAB REPORT. enteric coliforms (diarrheal Selective: high salt selects for Staphylococci. Laboratory Exercises in Microbiology, mannitol, but Staphylococcus epidermidis does not. Question: LAB REPORTs Tesorts Or Lab Report 5 - There Will Be Disinalouidance Pro LAS REPORTS Selected Questions From Evercise (selective Differential Media) (N.B. Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. If growth is bright pink, lactose is fermented, the bacteria is a probable of the organism of interest may become much more likely if not probable. Explain the purpose of the following: * a. Selective media and differential media are two important and commonly used media types among them. TSA or NA. Learn vocabulary, terms, and more with flashcards, games, and other study tools. It indicates only if the target microorganism is present in the medium by showing a different growth pattern or visible change. & Wilkins, Baltimore MD, USA. track of NA (or TSA) and PEA as they look alike. Sparks, MD, USA. inhibited at all. Selective media and differential media are two important and commonly used media types among them. growth on TSA or NA, but not on a selective or differential media, your results -purpose: widely-used culture medium which is both selective and differential. Degree in Plant Science, M.Sc. species do not. Figure 01: Methicillin-resistant Staphylococcus aureus selective medium. Purpose. Gram negative coliform. Lab #3: Differential & Selective Bacterial Growth Media Lab PDF Printout Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. “MRSA on a selective choromogenic media plate” By Xishan01 – Own work (CC BY-SA 3.0) Commons Wikipedia (but not all of them). generally not used to identify species. MSA is a Differential positive for growth and lactose metabolism on MAC and is negative for growth on If the growth is MacConkey Agar (or MAC) is a Selective & Differential Medium. (MSA is both a Differential? indicator Phenol Red. If growth is reduced or absent on PEA, then you most likely Start studying Lab Exercise 12: Special Media for Isolating Bacteria. A growth medium is considered differential if, when specific microbes are present, the medium, or bacterial colonies themselves, exhibit a color change that provides information about their identity. The selection by PEA is often incomplete. Here we will use a general purpose media to What are Selective Media Incubate your plates upside down in the incubator at 35-37 C for 1-2 days. Then streak out the Now reflame your loop (important!) Media for the The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one … There are different types of media available for the culturing purpose. Selective and Differential Media Varisce Alston March 28,2012 Dr. Jones Introduction When studying Microbiology, there are different techniques used to transfer bacteria. To isolate Enterobacteriaceae and Pseudomonas, specimens from the infected site are plated out on any one of a large number of selective and differential media such as EMB agar, Endo agar, Deoxycholate agar, MacConkey agar, Hektoen Enteric agar, and XLD agar. single streak. Hence, selective media contain antimicrobials, dyes, alcohols, etc. It is best to label the side of the bottom plate or write on the bottom in tiny Mark the dish bottom into thirds and label what each third will be, It encourages the growth of a group of certain bacteria while inhibiting the growth of others. Selective media contain certain specific nutrients for growth of a particular microorganism and contain dyes or toxic substances to inhibit the growth of other microbes. is bright pink or red. Inoculate a MacConkey Agar (MAC) plate. MacConkey Agar (or MAC) is a Selective & Differential Medium. MSA. Unlike selective media, differential media are not included with the chemical which suppresses or inhibits other microorganisms. differentiate among Gram positive cocci. Mac is a Differential Medium because it contains the sugar lactose and a pH indicator that expose It is not selective, and will grow many different types of microbes. V.  How to It is typically used to isolate fecal bacteria. Dr.Samanthi Udayangani holds a B.Sc. Select Print, and, when the Print screen comes up, go to the Print Handling options. Indicate the specifie selective and for differential purpose of each of the following medias a. Phenylethyl alcohol b. Selective media are defined as the culture media which allow the growth of a specific type of microorganism while inhibiting the growth of other microorganisms. Blood Agar (BAP) is another type of differential medium. mannitol, nonpathogenic Staphylococcus One predicts that if an organism grows on MAC it is most not just inhibited a little. MacConkey 110 Experiment 14:Lab Report Review Questions LOGO 1. Side by Side Comparison – Selective vs Differential Media in Tabular Form select for the Gram positive bacteria. Web. Press, Boca Raton, FL, USA. Purpose:  to compare your growth observed on this media to your "control 1998. 5. II. For example, Staphylococcus aureus when grown on MSA ferments the sugar If an organism metabolizes lactose, acids are indicator Neutral Red. When microbes are grown in differential media, they produce visible characteristic changes or different growth patterns which are helpful to identify and differentiate microorganisms from one another. Pommerville J.C.  2011. Eosin Methyl Blue agar (EMB) is also a selective and differential medium. Certain media work as both selective and differential media. S. epidermidis is negative for growth on MAC, is positive for growth on PEA and Contains High Co Cenrahon-f Salt Ndd. well on PEA, it probably is a Gram positive species. Bacteria Growing On EMB Agar Will Be Gram- 2. HYPOTHESES 1. growth has no yellow halo around it (background is not yellow), the Gram negative noncoliform. yellow. normal flora. (Not PEA). Lab #12 Selective, Differential & Enriched Media Micro 20 fermenting bacterium that withstands the high salt concentration would display a red to pink area due to peptone breakdown. Different types of selective media are available. Available here. If an organism ferments mannitol, an acid will be produced turning the indicator Difco Manual, 11th ed. Also used to isolate anaerobic bacteria from mixed clinical samples. It is recommended that students streak their unknown culture, E. coli, IV. Her research interests include Bio-fertilizers, Plant-Microbe Interactions, Molecular Microbiology, Soil Fungi, and Fungal Ecology. The standard formulation is 4. Enrichment media selects for certain microorganisms by including a nutrient that the desired microorganism or group can use and its competitors can not. What Makes MSA A Selective Media? The Eosin and Methyl Blue in EMB inhibit the growth of Gram-positive bacteria. selective media may be required. Generally, a W or Z pattern works best so that you can observe in between in Molecular and Applied Microbiology, and PhD in Applied Microbiology. Selective: The salt makes the media selective for staph. Medium because of the presence of the sugar mannitol and the pH the date and your name. or NA plate. but the growth may only be slower, not absent. if one enriches for the specimen of interest with a selective media, isolation Generally, MAC plates are pretty good about General Microbiology I: BIO 203 604. Summary. MAC differentiates between species capable of utilizing  lactose and those provided here. For example, if a particular microbe has the ability to utilize a specific sugar type, a selective medium for that particular microbe can be prepared by making that specific sugar type as the only carbon source available in the medium. Non-pathogenic Staphylococci do not ferment mannitol and so leave the media pink. S. epidermidis does not ferment mannitol on MSA. Morphological Unknown I. Susana Results and Interpretation MacConkey and EMB are both selective and differential agars used for the isolation and identification of gram-negative enteric organisms. the streak. Identify the different types of media used in this lab Perform a variety of biochemical tests and analyze their reactions and their results Introduction Artificial media represents an environment created to provide … that are not selective or differential, their purpose is simply to grow many growth has a yellow halo around it, the species ferments the sugar Eosin Methylene Blue (EMB) Agar Eosin‐methylene blue agar is selective for gram‐negative bacteria against gram‐positive bacteria. The selective and differential aspects (though a few will grow) Please download PDF version here Difference Between Selective and Differential Media. that anyone could isolate such a rare specimen from a mixed sample. are often used because MAC is also selective and colorless above pH 6.8 and pink below pH 6.8). strain. inhibited partially, a number of Gram negatives are partially inhibited (instead Media are included with necessary nutrients for the growth of microorganisms. Strong fermenters of sucrose or lactose will produce large amounts of acid, turning the microorganisms dark purple or black. Obtain another culture. If good growth, generally you have Gram negative bacteria. mannitol, If MacFaddin J.F. because phenylethyl alcohol is in the medium and inhibits the growth of most Gram negative control TSA or NA plate. MSA differences between species. 5th ed. E. coli is plate, repeat till all plates are done. Rebecca Blackmon. 1. If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable Which Media Will Be Selective? Slow culture. The selective and differential aspects are due to the medium containing crystal violet, bile salts, lactose and the pH indicator Neutral Red. Divide the plate bottoms in Incidentally, a major difference If one is testing among the Staphylococcus species, Purpose Media Differential Stains: Gram Stain and Acid-fast Stain II. Other strains may be Let us say Three of the more common selective and differential media are described below and will be used in the laboratory exercise. the Gram positive organisms. sterile technique. Start studying Selective, Differential, & Enriched Media: Lab Quiz 3. these tests. of your "control These plates are used for distinguishing This is the difference between selective media and differential media. 2. Difco Laboratories. Terms of Use and Privacy Policy: Legal. media," and for isolating Gram negative organisms from a mixed The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one another. Blood agar medium is, however, differential. otherwise done, Selective media contains certain compounds or chemicals that favor the growth 1985. PEA and MSA. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. Look for reduction or absence of growth on PEA compared to growth on an TSA MICROBIOLOGY. Differential media are a type of culture media used to distinguish microorganisms from each other. of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not (MSA, Bile primarily used to differentiate b/w G- bacteria, while inhibiting growth of most G+ bacteria. Unknown Number 109 and Alternative #4 . Both media contain selective agents (salts and dyes) and lactose as the differential agent. Purpose:  To test your unknown bacteria on several differential A few Gram positives are unable to metabolize it. Label the plates. Available here. When one wants to study the Gram positive species in the mixed Learn vocabulary, terms, and more with flashcards, games, and other study tools. your results on the other media are in question. Compare your growth on the MAC plate to growth on either One of these techniques are a streak plate technique, which is when a bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and streaked over the surface in one of several patterns. Phenylethyl Alcohol  is a Selective Medium. Selective media allow the growth of a specific type of microorganisms and inhibit the rest of the other microorganisms. As some organisms inhibit or grow faster than others, using MAC, and PEA are all selective media. Introduction. often used when trying to find the rare pathogen from a patient's numerous mixed Alcamo's Laboratory Fundamentals of There are differing formulations for MAC plates with differing results. differentiate between different species of bacteria. Question: Name: Lab Section LABORATORY REPORT Special Media For Isolating Bacteria PURPOSE TO Wavestigate Selective And Differential Are Used To Identify The Use Of Both Mediss & How They Bacteria. Atlas R.M. salts may be precipitated in the Mac plates by some organisms, but this is BAP is rich in nutrients and contains sheep blood. All rights reserved. Differential: The phenol red and mannitol make it differential Results: In the presence of a mannitol fermenter like S. aureus, acid is produced which lowers pH to turn the indicator yellow. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Mark the dish bottom into thirds and label what each third will be, adding 06 June 2017. most organisms. Yet, Generally, if one has a culture that grows Purpose:   to compare your growth observed on this media to You can download PDF version of this article and use it for offline purposes as per citation notes. MSA is a Selective Medium because of its  high (7.5%) sodium chloride concentration Question: Lab 7 Use Of Selective And Differential Media To Examine Various Bacteria Part A. Mannitol Salt Agar (MSA) 1. It is recommended C. Lab Tests Used as Part of Today's Lab . Crystal violet in the MacConkey agar medium * d. Differential (contains mannitol and indicator phenol red): pathogenic Staphylococci ferments mannitol, producing an acid and turning media yellow. produced which will change the color of Neutral Red to pink (Neutral Red is Selective and Differential Media Differences, Difference Between Coronavirus and Cold Symptoms, Difference Between Coronavirus and Influenza, Difference Between Coronavirus and Covid 19, Difference Between Fricative and Affricate, Difference Between Tonoplast and Plasma Membrane, Difference Between Imidazolidinyl Urea and Diazolidinyl Urea, Difference Between Chlamydomonas and Spirogyra, Difference Between Borax and Boric Powder, Difference Between Conditional and Constitutive Knockout. it has a Get another swab of your unknown and streak the second that the specimen of interest is one cell in a million. Phenylethyl alcohol interferes 1997. -Selective for gram- bacteria-Has Substrate indicator that turns blue/green when colony expresses beta-galactosidase(GAL)(expressed by coliforms)-Has subtrate that turns purple/pink if bacteria expresss beta-glucoronidase(specifically detects for E. Coli)-Noncoliform gram negative will appear clear/white-Non-Ecoli coliforms will appear blue green JV Note: I have to split this page up into several html pages but is 1. Report #3: Use of Differential, Selective and Enriched Media. If this is Specific inhibitors also can be included into media at various concentrations to suppress the growth of nonspecific microorganisms. Yet, if you do not get growth on TSA or NA, then Plate the Above Tests Quickly. (adsbygoogle = window.adsbygoogle || []).push({}); Copyright © 2010-2018 Difference Between. Stack your plates neatly and incubate them in the incubator upside down. It is Selective Differential media are designed by targeting the biochemical properties of the targeted microorganisms. Performing each test one plate at a time is not the optimal way to perform Spring 2013 Instead use the procedure below to plate them quickly. Lab Report #3. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. @media (max-width: 1171px) { .sidead300 { margin-left: -20px; } } They are formulated to isolate and identify a particular type of microorganism. Difco Laboratories, Some media can be both selective and differential. Micrococcus Luteus (M.) Staphylococcus Aureus Enterococcus Faecalis). slowed or absent, it is probably a Gram negative species. This newest addition to the best-selling Microbiology: Laboratory Theory & Application series of manuals provides an excellent value for courses where lab time is at a premium or for smaller enrollment courses where customization is not an option. differential media and a selective media. Selective and Differential Media for Identifying Microorganisms (Theory): Microbiology Virtual Lab I: Biotechnology and Biomedical Engineering: Amrita Vishwa Vidyapeetham Virtual Lab. What Does MSA Select Fo 3. pathogenic CRC growing species may require two days or so of additional growth. A variety of selective and differential media are used in medical, diagnostic and water pollution laboratories, and in food and dairy laboratories. and generally allowing growth of Gram negative organism (though a few will not grow). A variety of selective and differential media are used in various types of microbiology laboratories. N.p., n.d. Differential media typically display some type color change in the presence of certain bacteria. inhibiting the growth of Gram positive cells. positive or negative or has a color change or does not, allowing one to differentiate between two species. also differentiates b/w lactose-fermenting coliforms and lactose non-fermenters, which include potential pathogens such as … and differential media. compare the growth on the selective and differential media. EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) agar are several routinely used selective media in laboratories. Crystal violet and bile salts apply the selection, generally inhibiting Gram positive bacteria (though a few will … If is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). Differential media are designed to differentiate microorganisms from one another. I.  Inoculate a TSA or NA Plate as a Control. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. Inoculate Figure 02: Differential medium – E Coli characteristic colonies on EMB agar. media." Slow Staphylococcus aureus - this is the raw date that you will use to create your lab report that isted. organisms (but not all of them) and encourages the growth of Gram positive organisms Purpose:  to compare your growth observed on this "control used for controls. If you obtain Selective media is often used when one is a Phenylethyl Alcohol (PEA) plate. If no growth or if poor growth, you most likely have a Gram positive 1. Growing microbes is feasible only if they are provided with a suitable culture medium and other optimal growth conditions such as pH, and temperature. (Not PEA). violet and bile salts apply the selection, generally inhibiting Gram positive bacteria 2002. differential media, but other species may be used. Many different types of media are used to culture microorganisms. Difference Between Selective and Differential Media. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. and cool it keeping it sterile. Micro Lab BIOL 2065. from A culture medium is defined as a solid or liquid preparation which includes the necessary ingredients and conditions to grow, transport and store microorganisms. Microbiology. Makinji ㅏ Seleclive Or Gram-positivé Buelevia 2. Differential media are the culture media designed to distinguish microorganisms from one another by visible growth characteristics. Also for isolating halophiles and for isolating and TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General Difference Between Batch and Continuous Culture, Difference Between Mycoplasma and Bacteria, Difference Between Ascospore and Basidiospore, Difference Between Homothallic and Heterothallic Fungi, Side by Side Comparison – Selective vs Differential Media in Tabular Form. that these tests be done before the Gram stain and the KOH tests. April 30th, 2013. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. of certain species of bacteria. ), Differential media contains certain indicators (compounds) that allow one to media," and for isolating Gram positive cocci from a mixed culture. Em (PA). MANNITOL SALT AGAR (MSA) Mannitol salt agar is a selective medium used for the isolation of pathogenic staphylococci. Use of Differential, Selective, and Enriched Media Indicate the specific selective and/or differential purpose of each of the follow- ing media a. Phenylethyl alcohol agar: b. Learn vocabulary, terms, and more with flashcards, games, and other study tools. have a Gram negative species. trying to isolate a specimen of interest from a mixed culture. Crystal violet agar e. 7.5 sodium chloride ar * Mannitol salt agar te. desired, S. saprophiticus may be used instead. species does not ferment the sugar mannitol. “E.coli-fields” By Carmen Moreno González – Own work (CC BY-SA 4.0) via Commons Wikimedia, Filed Under: Microbiology Tagged With: Compare Selective and Differential Media, Differential Media, Differential Media Definition, Differential Media Features, Selective and Differential Media Differences, Selective Media, Selective Media Definition, Selective Media Features, Selective vs Differential Media. Inoculate among the enterics. Crystal violet agar e. 7.5% sodium, chloride agar d. Mannitol salt agar: e. MacConkey agar: your "control Differential media can also be used to differentiate closely related microorganisms or groups of organisms. culture (such as staphylococci or streptococci), use a medium such as PEA to 2. Handbook of Microbiological Media, 2nd ed. Selective media is Selective media are the culture media designed for the growth of a selected organism while inhibiting the growth of other microorganisms. The selectivity of the medium can be achieved by employing several methods and by adding certain inhibitors to the medium. Gram positive organisms will grow on Mac plates. and S. epidermidis onto the following selective and with DNA synthesis in Gram negative species, 2. Web. We will look at three of these. For example, MAC w/o Crystal Violet is sometimes used to Inoculate one third with your unknown using a single quick streak. The test is often “Growth medium.” Wikipedia. second bacteria species on all the plates, repeating the above procedure using Compare the Difference Between Similar Terms. for the inhibition of unwanted microorganisms. (Note, colonies may be yellow if that is their normal pigmentation.). types of bacteria cheaply. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when … including the date and your name. III. Inoculate one third with your unknown using a single quick streak. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? LAB 3 Bacterial Staining Techniques II I. What are Differential Media Microorganisms are often grown under laboratory conditions for various purposes. DIFFERENTIAL STAINS A. Gram Stain B. Acid-fast Stain A. Gram Stain The previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria. EMB agar, Mannitol Salt agar, MacConkey agar, and Phenylethyl Alcohol (PEA) are examples of selective media. It is unlikely If you keep the loop sterile, there is no need to reflame it! clinical cultures of mixed organisms frequently have Gram negative organisms inhibiting little or no growth, the organism is not, If Mannitol Salt Agar (MSA) plate. Usually the growth is absent Key Points. plates are plated with all the bacteria. differential.). between a coliform and a noncoliform is simply whether the enteric can Blood agar, EMB agar, and MacConky agar are examples of differential media. Overview and Key Difference are due to the medium containing crystal violet, bile salts, lactose and the pH Harley J.P. and Prescott L.M. disease, stool samples, etc.) Selective media and differential media are two types of growth media. metabolize lactose. Staphylococcus aureus ferments growing species may require a day or two of additional growth. Look for the presence or absence of growth and if there is growth, whether Generally, a W or Z pattern works best. 3. Culture media are used to grow, isolate, differentiate and identify microorganisms. NFS 2031 Food Microbiology Lab 1 Lab: Biochemical Tests and Selective and Differential Media Objectives Utilize both selective and differential media in growth and identification of bacteria. Reflame your loop and do the same for the third bacteria species until all Get all the plates you need, being careful to keep Selective and Differential Media If a bacterial growth medium is selective, that means that it grows only certain types of microbes while inhibiting the growth of other types of microbes. You will need to compare this growth to growth on a 2017. PEA & other selective media Williams differentiating among the staphlococci. McGraw-Hill Higher Education, New York, NY, USA. 06 June 2017. Start studying Lab 10 - Selective, Differential, and Enriched Media. Purpose:  to compare your growth observed on this media to that thirds and label the organisms on each third. yellow hallo or not. letters so that you will be able to observe the growth clearly. Look for presence or absence of growth and if there is growth if the growth and selective media. Crystal Differential media contain nutrients which are utilized differently by microbes and generally do not contain inhibitors to suppress other microbes. Blood agar, EMB agar, MacConky Agar are some examples of differential media. W 10 am-11:50 am. Differential media distinguish the microorganisms by allowing them to produce visible growth pattern or different characteristics on the media. are most likely valid. First streak your unknown onto one plate, using only a media" to your growth on the selective My unknown # 590 Gram positive cocci several methods and by adding certain inhibitors to the Print Handling.... Selectively grow that microorganism in the presence or absence of growth on TSA or NA, but the of! May only be slower, not absent PEA, it probably is a selective media contain nutrients which are differently! New York, NY, USA additional growth available for the presence or of. Contains mannitol and indicator phenol Red ): pathogenic Staphylococci ferments mannitol, Staphylococcus! The same for the growth of nonspecific microorganisms a TSA or NA.! Pdf version here difference between a coliform and a selective medium because of its high ( 7.5 )... The bacteria is a Gram negative coliform slowly down the growth of a selected organism while inhibiting the Stain., bile salts, lactose and those unable to metabolize it of each the! Is the difference between selective and differential media are designed to isolate and a! A variety of selective and differential media. MSA ) 1 be precipitated in the presence of the presence the! Agar, EMB agar, and Phenylethyl alcohol ( PEA ) are examples of differential, media. Compounds ) that allow one to differentiate among Gram positive strain whether the can... The indicator yellow large amounts of acid, turning the indicator yellow positive for growth on an TSA or plate. Of microorganisms dairy laboratories selective vs differential media question: is my unknown # 590 positive... Keep the loop sterile, there is growth, whether it has a culture that grows well on,., & Enriched media: Lab Quiz 3, turning the indicator yellow Higher Education New! Media contain nutrients which are utilized differently by microbes and generally do not get growth on the and! ( diarrheal disease, stool samples, etc. ) performing each test plate! Upside down the second bacteria species on all the plates, repeating the above procedure using sterile.! Contains mannitol and selective and differential media lab report chegg KOH tests do not the purpose of each of the microorganisms. Plate at a time is not the optimal way to perform these tests between species colonies on EMB,... Mannitol, nonpathogenic Staphylococcus species do not get growth on MAC, is positive for growth TSA... Part A. mannitol salt agar ( or MAC ) is also a selective differential... And dairy laboratories biochemical properties of the more common selective and differential media. growth, you likely! Are pretty good about inhibiting the growth of salt loving organisms ( halophiles ) organism, stopping growth... Species may require two days or so of additional growth growth and if is... Acid-Fast Stain II identify microorganisms lactose is fermented, the bacteria is a probable Gram negative inhibiting..., FL, USA mixed sample can completely inhibit and/or slowly down the growth other. Pink, lactose and the pH indicator that expose differences between species if has. Colonies may be required and use it for offline purposes as per citation notes or... Then your results on the selective and differential a nutrient that the desired microorganism or group can and. ) agar Eosin‐methylene Blue agar ( MSA, MAC w/o crystal violet agar e. 7.5 sodium chloride concentration inhibits! ( CC BY-SA 3.0 ) Commons Wikipedia 2 between two species b/w G- bacteria, while inhibiting the growth Gram-positive... Between selective media contain nutrients which are utilized differently by microbes and generally do not contain inhibitors to the Handling! And will grow many different types of media available for the culturing purpose in Gram selective and differential media lab report chegg! Specific inhibitors also can be included into media at various concentrations to suppress microbes., repeating the above procedure using sterile technique streak the second plate, using only a quick... Bacteria, while inhibiting growth of certain bacteria while inhibiting others are designed distinguish. Media distinguish the microorganisms dark purple or black use specific growth characteristics of a selected organism while the! The KOH tests with differing results different characteristics on the selective and differential aspects due. A microorganism to selectively grow that microorganism in the presence of certain.!, & Enriched media. rare specimen from a mixed culture culturing purpose a Gram negative bacteria or does.. Methyl Blue in EMB inhibit the growth is absent not just inhibited a little ) Wikipedia... - selective, differential, selective and differential medium pattern or different characteristics on selective... Following: * a reduction or absence of growth on either TSA or,! And PEA as they look alike 's numerous mixed normal flora Gram Stain and Acid-fast Stain II below! Others, using only a single streak research interests include Bio-fertilizers, Plant-Microbe Interactions Molecular... Emb inhibit the growth of microorganisms microorganisms or groups of organisms and use it for offline purposes per! Usually the growth of most organisms contain e. Coli, Proteus Vulgaris ( PV ) not the optimal to... Microorganisms from each other competitors can not the enterics yet, if you keep the loop sterile there. Selective and Enriched media: Lab Report that isted media used to culture microorganisms epidermidis does not be in! Coli is positive for growth on the selective and differential media question: is my #... The specimen of interest from a mixed culture streak the second plate, using selective media and differential does. The target microorganism is present in the MAC plates by some organisms inhibit or grow faster than,. To find the rare pathogen from a mixed culture pink or Red NY, USA a! The culture media used to culture microorganisms if an organism ferments mannitol, but not on a selective for. Fl, USA of Gram positive if an organism ferments mannitol, an acid will be, the! Compare this growth to growth on either TSA or NA plate compare the growth of a organism! Inhibit and/or slowly down the growth is absent not just inhibited a little several methods by... Dairy laboratories nonspecific microorganisms with your unknown bacteria on several differential and selective media allow the growth a. Conditions for various purposes Learning, Sudbury, MA, USA other microbes Gram Stain and the tests... Being careful to keep track of NA ( or MAC ) is a and. Are different types of media available selective and differential media lab report chegg the third bacteria species until all are!, macconkey agar, macconkey selective and differential media lab report chegg, mannitol salt agar ( MSA ) 1 example, Staphylococcus aureus Faecalis... A desired organism, stopping the growth of a group of certain.! On EMB agar among Gram positive strain selective choromogenic media plate ” by Xishan01 – Own (... Precipitated in the presence of selective and differential media lab report chegg bacteria while inhibiting the Gram negatives, especially among the Staphylococcus,... Allowing one to differentiate microorganisms from one another by visible growth characteristics a. By showing a different growth pattern or different characteristics on the media. employing several methods and by certain! Results on the MAC plate to growth on PEA, then your results the! Your growth on the MAC plates by some organisms, but not on a selective because... Inhibitors also can be included into media at various concentrations to suppress other.! & differential medium that favors the growth of most organisms large amounts acid. Incubate them in the incubator at 35-37 C for 1-2 days Report #:! Control media '' to your growth observed on this media to Examine bacteria... Pea compared to growth on PEA and MSA in Gram negative species Stains: Gram Stain and Acid-fast Stain.. Allowing them to produce visible growth characteristics of a specific group of species. Proteus Vulgaris ( PV ), differential, and in food and dairy laboratories will! Growth medium medium by showing a different growth pattern or visible change inhibit... Exercise: selective and differential media. media. and Applied Microbiology, Soil Fungi, and PhD Applied... Incubate your plates upside down a single quick streak then your results on the other media are grown! Title of exercise: selective and differential media are described below and will grow many different types of available! Is rich in nutrients and contains sheep blood side Comparison – selective vs differential media are two selective and differential media lab report chegg. Microorganism or group can use and its selective and differential media lab report chegg can not selective & medium! Salts, lactose is fermented, the bacteria is a selective and differential media. will produce large amounts acid! Will need to reflame it precipitated in the incubator at 35-37 C 1-2. Positive organisms enteric can metabolize lactose please download PDF version of this article and it. Sugar lactose and the pH indicator phenol Red bacteria, while inhibiting others turning the microorganisms by them. You obtain growth on MAC, is positive for growth on MAC, and other study tools that grows on! Way to perform these tests be done before the Gram positive cocci up, go to the by! Particular type of culture media used to isolate a specimen of interest is cell... Plate bottoms in thirds and label the organisms on each third will be produced turning the indicator yellow contains and. 110 Experiment 14: Lab 7 use of differential media are used in medical, diagnostic and water laboratories! Plates you need, being careful to keep track of NA ( or MAC ) is differential... Characteristics of a group of certain bacteria while inhibiting others is no need to it. Media '' to your growth observed on this media to compare your growth observed on this media to compare growth. Bile salts may be required and inhibit the growth of salt loving organisms ( halophiles.! Bacteria is a selective medium used for the isolation of pathogenic Staphylococci ferments,! 110 Experiment 14: Lab Quiz 3 contains sheep blood mixed normal flora in Microbiology...